Indirect Hemagglutination Test for Chlamydial Antibodies
نویسندگان
چکیده
منابع مشابه
Indirect hemagglutination test for chlamydial antibodies.
An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the com...
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Purified chlamydial elementary bodies were subjected to sonic lysis to produce soluble antigen for passive hemagglutination (PHA). PHA was more sensitive than complement fixation (CF) in detecting antibody in hyperimmunized rabbits, lambs, and calves. Both CF and PHA tests were used to monitor antibody responses in experimentally infected pregnant cows. All animals responded with rises in titer...
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In an effort to obtain the flexibility and ease of performance of a rapid, serological test for detection of cytomegalovirus antibody, the indirect hemagglutination (IHA) technique was investigated by using a microserological system. Antigens were prepared from tissue cultures of infected human fibroblasts. The specificity of the cytomegalovirus antibody response detected by the IHA test correl...
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An indirect hemagglutination test has been adapted for use with varicella-zoster serology. Antigen was made in infected WI-38 human diploid cell line. The indirect hemagglutination antigen seemed to be a subunit of the virus particle. The antibodies measured in the test are well correlated with neutralizing and complement-fixing antibodies in cases of zoster but not in cases of varicella.
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An indirect hemagglutination test for a seroepidemiological survey of Streptococcus pyogenes infection was standardized. This is an improved modification of the indirect hemagglutination test which utilizes an unstable reagent prepared with fresh blood cells. Two types of bacterial antigens represented by extracellular products and purified streptolysin O were assayed, but only the former antig...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1972
ISSN: 0003-6919
DOI: 10.1128/am.24.1.22-25.1972